FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_278_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_278_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12290015
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA17000.011.40226861
GGCGGTA19400.09.2766659
GTCTAAG23600.08.48258344
GCGGTAC24950.07.906585760
CGCGGTA7604.913654E-77.70240717
ACTAGGC14550.07.57157611
ACGGGCG26800.07.49416256
TATACGC6603.6192472E-57.30424367
GGGCGGT27150.07.26709358
GCGTATA7603.993706E-67.25449139
ACACACG31000.07.246397552
CTCGATT16050.07.095048449
CCGCTTA10553.6476195E-86.879190453
TCTCGAT16150.06.83744648
ACACGGG32850.06.63090354
GATTCCG16251.8189894E-126.592935652
GAATCCG16251.8189894E-126.5695124
TCGTTGA18100.06.49188552
CGATAAT8652.4859886E-56.4113551
TCGATTC18350.06.394429750