FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_278_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_278_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12290015
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG6050.014.25500231
GTCCGAT15250.012.1381331
CGACCGT6700.011.8422932
GTCGGTT9200.011.7684611
GTCGCTT15800.011.2735041
GTCGGAT17000.011.0940641
GTCGAAT10900.010.8942731
GTCGGGA18350.010.6585431
GTCGTTT20950.010.5027341
GTTCGCG3004.5327062E-410.4777281
CGATCGG8900.010.4635441
GTCCGGA16050.010.0098751
CCCGAAT13050.09.9023221
GTCGTGA10700.09.7922691
GTCGGAA18950.09.7681541
GTCGTTA11550.09.6764011
GTTCGAC8651.8189894E-129.57145129
CCCGTAT8801.8189894E-129.5252071
GCCTGAT36700.09.4213911
CCCGCAT16700.09.4111321