FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_277_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_277_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3338442
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCCG5800.012.50904452
CGATTCC6600.011.51297551
CTCGATT4803.3833203E-911.50329549
GTCCGTT2450.00105301911.3196861
TCGATTC6008.185452E-1110.92862250
ATTCCGT5805.14774E-1010.72203753
CGGTACA5007.229755E-810.38508261
TTCTCGA5452.4463588E-810.13377747
GGCGGTA4957.0329224E-79.78961959
TCTCGAT6051.19216566E-89.69699348
TAAGGCG2850.00384624959.68698548
GCGGTAC6302.197703E-89.34023660
TTTCTCG6655.529546E-88.82419646
ATTCCGG3650.00309309048.49131317
CCCGGAC8253.0486262E-98.34648611
CCGGACA7851.1112206E-88.33294612
CGTGGGT7554.591311E-88.2571557
CGGACAC9701.10958354E-108.16339313
ACACACG7252.1914639E-78.10109452
GCCCGGA11103.6379788E-128.06499310