FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_277_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_277_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3338442
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG1952.161687E-819.45863231
CGATCGG2251.11243935E-716.8626341
GTCCGAT4400.015.0761721
CGACCGT2657.117087E-714.31861632
CGTGCGA2901.2653436E-714.27244737
GATCGGC3152.2509994E-814.23468942
GCGATCG2708.797779E-714.05219340
ATTCGAA2951.5586193E-714.0309618
GTCGCAA2052.2102853E-413.6246791
GTTCGAC2557.5166245E-613.527390529
GTCGAGA2456.916132E-512.8252721
TTCGACC3306.091068E-712.54358130
CGGATTA2255.3761E-412.26373264
GGCCGTT3501.0726071E-611.9702541
TACGACG2050.003735032911.7780239
TTCGAAT3257.028586E-611.67447959
GTCGGCT5105.8935257E-1011.6377461
GTAAGCG2100.004471584711.4975938
GTCGGAT4308.999996E-811.3671011
ATCGGCT4259.0563844E-811.36198743