FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_276_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_276_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14875611
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT17200.09.43005549
GCGGTAC23250.08.33674460
GATTCCG19350.07.857264552
CGGTACA22950.07.843012361
GGCGGTA20800.07.65471259
TCGATTC20150.07.365034650
GTCGAAC7754.9079645E-67.15496871
TTCTCGA20350.06.953879447
ATTCCGT21950.06.92656453
GTTTAAT75350.06.89917951
TCTCGAT20600.06.868492648
GTTAGAT42350.06.5467541
GTTTGAT93500.06.5236481
TTAATCG11804.8123184E-86.4199413
TCACCCG27950.06.1599693
GGAAAAT145400.06.14956951
CGGGAAT20500.06.08608631
CGATTCC25550.05.948302351
ACACACG34450.05.9178352
GTGAAAT116550.05.91739031