FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_276_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_276_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14875611
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAT11800.014.2044811
GTCGGTT12250.013.3976291
TCGACCG8650.013.16046231
CCCGTAT13600.013.0947571
GTCCGAT14550.012.7197631
CGACCGT9750.012.02950132
GTCCGTT17700.011.8370681
GTTCGAC10400.010.94596129
GTCGTTT24500.010.8321251
GTCGTAT14950.010.7444151
CGCGTAT2600.001628426310.7444151
TCGACGC11700.010.61269643
GTCGGAA19800.010.4052611
GGCGTAT14550.010.0798121
CTCGTAT19100.010.055311
GTCGAGA8151.8189894E-129.854541
GTCCGAA14650.09.7726511
GTCGGAT17700.09.6669391
GTCCGCA21000.09.6443921
TTCGACC12550.09.620530