FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_275_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_275_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11265358
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG7850.018.8962231
CGACCGT8900.016.6668932
GTTCGAC9800.015.84026929
CGCGTAT2352.8308841E-614.8657061
CGATCGG9100.013.26591641
TCGACGC11950.012.98838643
TTCGACC11500.012.89872430
AGTTCGA12850.012.34760228
GCGATCG9100.012.12889140
CGCGTAA5652.7284841E-1111.5991867
GCCGTAT9700.011.5247541
GTCGCTT16150.011.4645431
GTCGATT11000.011.433081
TCTCGAC13800.011.24718941
TCGCGTA5855.2750693E-1111.20262766
GTCCGAT12200.011.1675581
AAGTTCG14050.011.04774727
GTCCGCT13650.011.0049791
GTCCGAA11200.010.9170041
GTCCGTT12500.010.8995361