FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_274_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_274_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3353362
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA2250.00756558810.71333717
CTCGATT4401.898931E-59.41233849
TAGCCGA3000.00585257179.20606438
CGTAATC3450.0018558238.983232519
TTCTCGA4653.640792E-58.90683147
GTCGAGC3150.0084588358.80203151
GATTCCG5251.5932905E-58.55731752
ACTAGGC3650.00309746888.48998710
TACATCG6503.816458E-78.4757283
TCTCGAT4906.732276E-58.45189548
ACTTTCG3700.0034994768.3761325
TAGACCC4150.00106406658.2970223
TCGATTC5008.5147454E-58.28360150
GTTCGAT4550.00247145147.6171421
CGCCATA4150.00982125857.466317724
ACCCATG12002.3646862E-117.46096236
AATTCAC13900.07.43206025
ATTCCGT5603.120269E-47.40537153
TTCGGCG4700.0036230217.32511229
GTTTAAT15650.07.30807641