FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_274_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_274_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3353362
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGA1709.13606E-414.20298849
GTCGGGA5250.013.3004391
GCCGATT3507.63921E-812.9679271
CGATCGG2751.6379156E-512.5421541
GTCGATT5104.5474735E-1112.3224651
GCGATCG2801.970749E-512.31818340
CTCGACA3205.165315E-612.0015681
GTCGGTA4409.6915755E-911.9023821
TCGACAT3205.831116E-611.8718292
CGTGCGA2651.6120962E-411.71389437
GTCGGTT2701.7127406E-411.6378851
GTCGCAA2100.004084480511.6378831
TCGAATA3054.6632726E-511.3233542
GCCGTAT3401.0143998E-511.2955941
GTTCGGA3752.4613964E-611.172371
ATTCGAA3105.5750934E-511.1265988
GTCCGAT4105.9670674E-711.0701831
GATCGGC3451.3653667E-510.99710242
GTCCGTA5107.2868716E-910.9533021
GTCGAAT3206.80605E-510.9105161