FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_273_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_273_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18767526
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT23350.010.0506149
GATTCCG25750.09.39450552
ATTCCGT25250.09.03307553
TCGATTC26000.09.02691950
GTAGTCG16450.08.84448557
GTCGATT10750.08.7062461
TCTCGAT26900.08.5959348
GGCGGTA28050.08.14433759
TTCTCGA29300.08.12775247
CGATTCC32100.07.96391251
TCACCCG36550.07.53645853
CGGTACA29900.07.52548661
CGCGGTA10801.0768417E-97.332958717
AATCGTC28650.07.33175
GTTTATT117850.06.9121631
CAATCGG30500.06.8873384
GTCTAAG35200.06.863314644
CGGTAAT12051.4661055E-96.858029419
TCGACTG24700.06.8312077
CGGGAAT32550.06.8156091