FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_273_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_273_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18767526
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG9900.018.81597331
CGACCGT11600.016.05846232
GTTCGAC13500.013.79838329
GTCGGTT14150.012.0970381
CGATCGG13550.011.45460441
TCGCGTA7450.011.11145866
TTCGACC17200.011.03068330
TCGACGC17050.010.92383343
GCGATCG14950.010.84337940
GTCCGTT22150.010.724441
CGCGAGT8750.010.64293543
GTCGGAT26950.010.4994281
GTCCGAT24300.010.4943611
GTCCGCT23650.010.48737051
AAGTTCG18900.010.40254427
CCCGAAT23000.010.3281011
GCCGTAT19300.010.3170831
GTCGCAT14650.010.2534561
GTCGTTT31850.010.2002991
TCGCGAG9850.010.15473542