FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_250_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_250_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14705126
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT17400.013.28975449
GATTCCG19650.011.78276852
TCTCGAT19550.011.65168148
TCGATTC22600.010.07976950
ATTCCGT23400.09.89450453
TTCTCGA23100.09.56311547
CGGTACA22200.09.1989661
TCACCCG28250.08.7759493
CCGGACA28600.08.54643812
GGCGGTA23100.08.54029659
CGATTCC27600.08.38558451
CGTGGGT29650.08.29400857
TTTCTCG29350.07.8795746
GTAGTCG13200.07.871873457
GTCTAAG29450.07.61819444
CCCGGAC32350.07.237072511
CTCACTA38100.07.14026937
ACGGACA42300.07.082385518
AACTCAC56900.07.0808615
ATCGACT21550.07.03101256