FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_250_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_250_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14705126
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT11450.013.7261581
TCGACCG8650.012.76146331
CCCGTAT13200.012.170991
GTCGTAT14050.011.9318251
CGACCGT9750.011.32170832
CCCGAAT16400.010.8609621
GTCCGTT16100.010.8464121
GTCCGAT17900.010.3410551
CGCGAGT6051.1059456E-910.26186143
GTCGAAT13300.010.2412971
GTTCGAT23200.010.236771
GTCGCAT11200.09.97871
CGATCGG10100.09.90342941
GTCCGCT18600.09.7641041
CCGGTTT20050.09.5805461
GTCGATT17650.09.4981381
GTCCGAA14400.09.4589751
CCGGTAT14800.09.439311
GATCGCG5857.411472E-89.433537540
GTCCGGT22600.09.2722431