FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_249_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_249_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7824585
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG4150.016.62473531
CGACCGT4850.014.2252932
GTCGTAT6550.013.3263271
GTTCGAC5753.6379788E-1211.99872229
GTCCGGA10500.011.3058021
GTCGGAT10700.011.0944791
AAGTTCG7700.010.75127727
TCGACGC7101.8189894E-1210.687543
GTCGCTT10850.010.6193011
TCGGATA5907.003109E-1010.5361562
CCCTAGT6007.748895E-1010.4744931
CTCGTAT10050.010.422381
GTCGGTT6452.4920155E-1010.2850321
CCCGAAT8850.010.2575061
AGTTCGA7202.1827873E-1110.06052928
GTCCGCT8750.09.9757071
CCGGTAT8900.09.8075781
CCCGAAC9350.09.7089781
CCGGAAA6503.09592E-99.6687631
CTCCGTT17600.09.5222661