FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_248_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_248_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13312943
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAA3956.340281E-48.74143833
GGCGGTA15851.8189894E-126.768156559
GGAAAAT124550.06.56839751
GTTTTTT173950.06.5364291
CAATCGA10058.4667045E-76.51066834
ATCTAAG28100.06.4950346
GTTTATT91700.06.3886421
GTGAAAT106150.06.3353831
TTCGATA12057.282688E-86.28632742
CTCGAAC11207.361359E-76.19017741
GTTTAAT64500.06.18058061
GTTAAAT62050.06.14528561
CTCGATT14602.1082087E-96.145262749
CGGTACA18105.456968E-126.11876161
TACGTCG7550.0014078365.94549236
TCGATAC9501.0694012E-45.7993453
CTTGAAT69700.05.769211
AATCGAT12601.1036536E-65.73932275
GTTGAAT53300.05.7233011
GAAATCG26850.05.64275173