FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_248_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_248_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13312943
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT2259.665382E-817.0727941
GTCGGTT11500.011.8429861
GTCCGTT16400.011.7115191
GTCCGGT18350.011.2282061
GTCGCAT11000.011.1114251
GTCCGAT13150.010.8881111
GTCGGAT14600.010.76351451
GTCCGCA18250.010.7156761
GTCCGGA16500.010.582311
TCGACCG5959.151336E-99.85583531
GTCGCTT19050.09.7157281
GGCGTTT21700.09.6557481
GCCGTAT13500.09.5711121
CTCGTAT19350.09.5650961
GTCGAAT12100.09.5240791
GTCGGAA17300.09.4873781
GTCCGAA14000.09.4787261
CCCGAAT15250.09.3887641
GTCGTTT21750.09.3124331
GTCCAGT69600.09.2321541