FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_247_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_247_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6415919
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT9000.011.88769749
TCTCGAT9000.011.50422348
GATTCCG9250.011.20686952
TTCTCGA10500.010.18881747
TCGATTC10650.010.04696250
ATTCCGT11050.09.69402553
TACGCGA3050.00654301759.08223256
TCACCCG11450.09.0220393
TTACGCG3100.00752734078.92846755
TTTCTCG12400.08.62762746
ATTCTCG6201.7612238E-68.3314196
CGTGGGT12950.08.29255857
CGATTCC12200.08.21069451
CCGGACA13600.08.10056712
CCCGGAC13950.07.89769611
CGTCGTA5755.0125753E-57.80939251
GTTTAAT29400.07.7812981
CGGCCCG17100.07.65256268
CGGACAC16250.07.41513313
ACCCGGC15750.07.21527345