FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_247_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_247_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6415919
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG4051.8189894E-1215.33164531
CGACCGT4301.8189894E-1214.4402732
GTTCGAC4651.0913936E-1113.35336829
GTCCGCT9250.013.221431
GTCGCAT6100.012.602161
GTCGGAT7150.011.7289011
TCGCGTA3359.851645E-611.32578766
GTCGCAC7750.011.2717271
GTCGAAT5804.2382453E-1010.8441781
TTCGACC6402.3646862E-1110.78006330
TCGACGC5805.311449E-1010.70379843
CGATATA3551.8546436E-510.6973854
GTCGCGT4259.387659E-710.6882491
GCCTAAT10150.010.67204951
CGATCGG4854.8841684E-810.66709941
GGCCCGT6653.8198777E-1110.5089781
GTCCGGG11800.010.3642571
CGCGTAA3351.2252045E-410.2963367
GGCCTAT7201.6370905E-1110.1915191
GTCGGTT5552.6857379E-810.0734711