FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_246_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_246_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4245942
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT5000.015.87320949
TCTCGAT5850.012.97698248
ATTCCGT6150.012.9253453
CGATTAG2150.00539178811.2127264
GATTCCG7100.011.19589352
TCGATTC6900.011.00300350
TACGATA7950.010.8268427
TCACCCG8050.010.6932273
CGGTACA6857.2759576E-1210.61260561
GTAGTCG9250.010.4858357
CGATTCC7950.010.42926551
CGATACG8150.010.13786929
GATACGG9000.09.94543730
CCCGGAC8101.8189894E-129.77531411
TCTATCG3209.348463E-49.6840533
ACCCGGC10700.09.6551365
ATCGTCC13300.09.5801216
GAATCGT12950.09.57384
ACGGAAT8701.8189894E-129.52433833
TTCTCGA7302.8921932E-109.45686947