FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_246_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_246_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4245942
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA2453.8198777E-1119.70952666
CGCGTAA2655.456968E-1219.52358867
TCGACCG3400.018.26266331
CGACCGT3500.017.74087332
GTCGCGT2401.2361852E-817.24583665
TCTAGCG1452.728381E-416.6521327
CGATCGG3857.2759576E-1215.22955141
GTCGGGA5350.015.0124341
CGCCTAT1708.3240255E-414.3789181
GTTCGAC4353.6379788E-1214.27426629
GCGATCG3951.5643309E-1013.97081840
GTCCGCT4404.9112714E-1113.4919061
CGTGCGA3901.9317667E-913.26555937
TCGACGC4457.4578566E-1113.17612843
TTCGACC5053.6379788E-1212.97874630
TCGCTCG3751.5847036E-812.87795448
ACGCCGA3255.063557E-712.73643748
CGACGAA6850.012.58686719
GTCGTTT8050.012.5799571
CCGGAAA3103.6159363E-612.3910481