FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_245_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_245_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11485751
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG4900.017.5998431
CGACCGT5100.017.58603732
GTTCGAC7100.013.11807829
GTCGGTT10650.011.8087471
TTCGACC8800.011.75989330
GTCGCAT9150.011.0720461
CCCGTAT9150.011.0720461
GTCCGAA10450.011.0318571
TCGCGTA4703.0764568E-811.00801666
GTCCGTT15600.010.9729251
GTCCGAT14700.010.9317941
GTCCGGA15900.010.5461781
TAGACGC10500.010.51182739
GTCGTTT19000.010.4802641
GTCCAGT56000.010.4802631
TCGACGC8650.010.36747243
GTCGGCT16000.010.2619251
GTTCGAT18050.09.8706081
GCCGTAT11050.09.8005491
CCGGTAT13950.09.7665541