FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_244_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_244_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10017003
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG3602.454624E-49.61089255
TACGCGA3300.00117679069.44377656
CTCGATT10751.8189894E-128.34736949
GTCGATT4905.95342E-47.77946331
TCGATTC12253.6379788E-127.607553550
GTCCGAT6008.17173E-57.5083611
TTCTCGA12901.8189894E-127.492053547
ACGCGAC5250.00124645087.25849857
GATTCCG12505.4569682E-117.187802352
CGGTACA13607.2759576E-127.127049461
GTTTATT67100.06.55894851
GTCGGTT6400.00138768946.497621
GTTCAAT26250.06.46874141
GGCGGTA14108.8039087E-106.38271859
CGGGAAT14159.313226E-106.36751461
CGCTAGA6550.00184467926.33570466
GTTTAAT47750.06.31391761
CGTCGTA7157.272664E-46.28090451
AATCGGG7157.5544347E-46.26117755
TTCAATC26950.06.2598652