FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_244_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_244_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10017003
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGA6100.013.7367331
GTTCGAC6200.012.79684729
TCGACCG5900.012.27818431
GTCGTTT16350.012.1719221
CGACCGT6300.012.04616832
GTCCGTA12350.011.8736531
GTCGGAT10900.011.8516081
GTCGCGA2952.9699877E-511.835321
GTCGCAT6900.011.6380651
GTCGGTT9800.011.4005541
GTCCGTT12000.010.4742581
GTCGGTA9500.010.29051
GTCCGAT9650.010.1305441
GTCGGCT14400.09.9408471
TTCGACC8001.8189894E-129.91755630
TCGCGAT3157.859402E-49.8693092
GCCCTAT17750.09.8349851
GTCGTAT10550.09.5972671
CCCGTAT10200.09.584291
GTCCAGT51700.09.5220541