FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_243_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_243_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11350328
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGTA16000.08.22034159
CGGTACA17300.08.00340561
GTTTAAT57050.07.1691961
GCGGTAC20000.07.09526460
GTTCAAT29950.06.71236041
GTCTAAG21650.06.69528744
CCGCTAG6400.00141982896.48448865
CTCGATT12351.6709237E-86.427052549
GTTGAAT48150.06.3347651
GTTCGAT13209.036739E-96.30204341
GTTTTAT92650.06.17280631
CGGGAAT15207.4396667E-106.15693041
GATTCCG13651.9699655E-86.075583552
GTTTATT73100.06.0218571
GTTAGAT29300.05.91488741
TTTAATC54150.05.84963662
GTTTTTT151650.05.82831
GTTAAAT55950.05.82333661
TCTCGAT13751.4936268E-75.77266248
ACACGGG26400.05.762025454