FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_243_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_243_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11350328
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT10000.016.7681771
CGACCGT6400.012.93603632
TCGACCG6500.012.7370231
GTCGGAT10900.011.5377361
GTCCCGT17650.011.083781
GTCCGTT14200.011.070541
CGAATCG3451.353534E-511.006323
GTTCGAC7600.010.89350229
CCCGTAT9650.010.8602181
GTCCGAA12200.010.5946481
GTCCAGT57550.010.5620581
GTCCGCT14050.010.4428151
CGATCGG7600.010.43818141
GGCCTAT12200.010.3083061
GGCGTTT17850.09.9810581
TCGCGTA4201.1025511E-59.85476866
GTCCGGA14450.09.6702291
GTCGCTT16350.09.614781
GCGATCG8651.8189894E-129.56990640
TCGACGC10100.09.56196743