FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_242_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_242_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19239117
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT24450.08.7515449
GATTCCG25400.08.29974152
TCTCGAT26650.08.2880948
CGGTACA28400.07.7995761
TTCTCGA28800.07.19051347
TCGATTC29000.07.021962650
TCACCCG35150.06.95517253
GTCTAAG34950.06.9126644
ATTCCGT30250.06.854791653
GACGTAA15051.4551915E-116.64356541
GTTTAAT92050.06.51512771
TCGACTG27650.06.4761517
GTTTGAT111400.06.47261
CGATTCC34200.06.363996551
GGCGGTA31600.06.352012659
GCGGTAC34950.06.33715360
ACTAGGC22200.06.203354411
GGGAAAT114250.06.1290871
GTTTTAT155050.06.08131841
GTAGTCG15451.1168595E-96.05307157