FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_242_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_242_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19239117
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG11100.018.64659931
CGACCGT11800.017.54044532
GTTCGAC14400.014.61297629
TCGACGC16900.013.06185343
GTCCGCT25450.012.0788971
GTCGGTT16300.011.7871131
TTCGACC18550.011.715692530
GTCGAAT16500.011.00911
AGTTCGA20700.010.831128
AAGTTCG20750.010.63899127
TCTCGAC21700.010.49051641
GCCGTAT21000.010.3134691
GTCGTTT30600.010.2743291
CGATCGG13650.09.85472741
GTCCGGA26300.09.6961541
TCGCGTA8550.09.68198866
CTCGACG23600.09.64594142
GCCGACC24050.09.60902165
GGCGTTT30250.09.5848451
GTCGTAA19000.09.5605331