FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_241_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_241_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17379215
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATT10950.09.4937821
CGGTACA23400.08.28515861
GTTTAAT80500.07.9205271
CGAACTA11457.2759576E-127.8183583
CTCGATT21200.07.488016649
TTGCGTA15550.07.30564816
GTTCGAT22500.07.0844711
GGCGGTA25100.07.0333759
TCTCGAT22600.07.024157548
ACTAGGC22550.07.023829510
GTCGATA6906.5147586E-56.98562672
CGAAAAT25050.06.63996271
CTTAGAT45650.06.45223671
GTTTATT104400.06.43922141
TACATCG31700.06.4082643
GAATCGT27150.06.34182264
TTCTCGA25100.06.325762347
CTCACTA46050.06.2810337
GTTTTTT219300.06.27312561
GCGTATA9351.2550254E-56.265634539