FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_241_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_241_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17379215
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG7600.013.6168531
GTCGGTT14900.012.4210561
CGACCGT9100.011.75139132
GTCGCTT23200.011.74021
GTCCAGT82300.011.1589881
GTCGGAT21450.010.7444831
GTCGAGA13850.010.5893281
GTCCGAT22550.010.3752161
GTCCGGT28100.010.31432151
GTCCGAA17350.010.2645421
GTCGAAT16100.09.977021
GTCGGCT27300.09.7211991
CGATCGG10750.09.62560541
GTCGTTT30200.09.5970991
GTCCGTT22300.09.39541
GCCGTAT20350.09.2661271
CCCGAAT22350.09.2181421
CGCGTAT3050.00610537979.1592321
CCGGCTA13600.09.13022237
GTCCAAT52850.09.1180721