FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_224_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_224_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15842389
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT17850.08.89325749
CCGCTAG10001.9281288E-107.9543265
GGCGGTA24900.07.228709759
GATTCCG22950.07.227319252
TCTCGAT22050.07.199303648
TCGATTC23150.07.155880550
ATTCCGT23100.07.03079853
CGGTACA25650.06.88289861
GTTTAAT75350.06.76106171
TTCTCGA23550.06.74158147
GTTAAAT73600.06.54512361
CGCTAGT7401.5672622E-46.531942433
GTCTAAG27500.06.526173644
GTTTATT100150.06.4017931
GGAAAAT142700.06.1929431
CGCTAGA12451.2676173E-76.11128766
GCGGTAC27950.06.06824160
GGGAAAT100050.06.0271641
GTTTGAC64600.05.95485641
GTTTTTT201450.05.93516351