FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_224_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_224_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15842389
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG9050.015.62832331
GTCGGTT14450.015.2281331
CGACCGT10150.013.59474532
CGCTAGT5300.013.1803861
CGATCGG10750.013.15466741
GTTCGAC12300.012.05980929
GTCGTTT25650.011.84693051
GCGATCG12100.011.68703440
GTCCGCT19300.011.4013751
TTCGACC14250.010.89368330
GTCGCGA4208.1062717E-710.8110531
GTCGGAA22750.010.7470831
GTCCGGA20400.010.6153791
GTCGCTT22600.010.3547671
TCGACGC14750.010.28867943
GTCGGAT16650.010.2791181
CCGGAAA14300.09.7700761
CGTGCGA13450.09.7446737
GCCGTAT15000.09.5469921
ATTCGAA15200.09.5305598