FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_223_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_223_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14060200
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA21100.08.52944761
GGCGGTA21150.08.18153559
GTTTAAT69050.07.9834711
CTCGATT15950.07.789493649
GCGGTAC24350.07.674576360
GTCTAAG26100.07.669613444
TCGATTC17850.07.3477850
GATTCCG18800.06.80069852
GTTCGAT16350.06.78563551
CGCGGTA6804.110748E-46.583408417
TAGCATA36900.06.53286179
TTCTCGA20100.06.525058347
GTGAAAT109100.06.4192591
GGAAAAT129850.06.40807531
GTTTTTT179000.06.2948971
TCTCGAT20400.06.259490548
ACGGGCG30050.06.221390756
TCACCCG27200.06.20344163
GTTTATT88550.06.10793351
GTTTTAT110850.06.06770321