FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_223_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_223_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14060200
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG7700.015.23183831
GTCGGAT13900.013.8234591
GTTCGAC9450.012.77616129
GTCGCAT12100.012.7038731
GTCCGTT16900.012.6099081
TCGCGTA5501.8189894E-1212.54270766
CGACCGT10200.012.1749332
GTCGCAA7800.012.093111
CGCGTAA6007.2759576E-1211.49752267
GTCCGCT17750.011.4155921
GTCCGGT21000.010.8134151
GTCCGTA15350.010.6969091
CGCGTAT2953.8712498E-410.6583341
GTCCGAT14500.010.6011621
GTCGGTT14400.010.4321741
GTCGCTT21200.010.381821
GTCGGGT21600.010.3513031
GTCGTTT23300.010.345751
TCGACGC12500.010.20940143
GTCGGTA13400.09.9071241