FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_222_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_222_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12728700
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCCG13500.09.21531352
TCGATTC15200.08.40140350
CTCGATT14350.08.17666649
GTAGTCG12900.07.786469557
GTTTAAT55900.07.75110531
ACTAGGC16500.07.720137611
CGATTCC18150.07.42304551
ACGCGAT6301.5152452E-47.14241658
GAATCGT21250.07.13067534
TCGCGAA6351.7251926E-47.067502533
GGAATCG22600.06.85578783
TAGTCGC14352.7284841E-116.75380458
AGTCGCC16650.06.65184459
GTTAAAT54200.06.58723831
ATCGCGA7351.4510167E-46.570707332
TCTCGAT16351.8189894E-126.543244448
GGAAAAT109850.06.43717861
CGGGAAT23350.06.38332561
GAATCGA12158.5377906E-86.23567344
ATTCCGT17803.6379788E-126.212570753