FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_222_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_222_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12728700
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG6000.016.67302131
CGACCGT7400.014.45098932
GTCGTAT13350.011.5105391
GTTCGAC9050.011.43510829
GTCGCTT18100.010.80522251
GTCCGTT15700.010.4549611
GTCGTTT22400.010.4460251
CCCGAAT15750.010.4217711
CGCGACT3005.09073E-410.34661814
GTCCAGT58650.010.1229051
TCGACGC10300.010.04585343
GTCGGCT19500.010.0294631
GTCGGTT11600.09.9352821
GTCGCGA2850.00348618379.8032341
GTCGCAT10700.09.7917821
CTCGTAT17000.09.6554651
GTCCGTA16300.09.6416021
GTCGGAA19300.09.5905341
TTCGACC11550.09.55730830
CCCGTAT9001.8189894E-129.3130721