FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_221_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_221_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4004936
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT4450.014.73878149
TCTCGAT4852.1827873E-1112.81146248
TCGATTC5105.4569682E-1112.18375550
ATTCCGT5501.6370905E-1111.9356253
GATTCCG5502.0372681E-1011.3074352
CCGCTTA3106.769235E-410.03078653
CGATTCC6756.9485395E-109.72142351
TTCTCGA6757.021299E-109.71570447
GTAGTCG4401.8197643E-59.44641257
CCGATTG3000.0057506329.22583155
ACTAGGC5654.63966E-79.14028411
TCACCCG7657.348717E-109.00283
TTCGTTC4603.2952295E-58.982782
TCAACCG5051.0210477E-58.8646393
TCGACTG5503.1628388E-68.7645787
CGAAAAT5755.2653413E-68.4394671
TTTCTCG7554.8657967E-88.22905546
ATCTAAG8801.1714292E-98.2167926
TTACCGA5502.9959634E-58.13772424
CAACTAG8152.1627784E-88.0271559