FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_221_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_221_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4004936
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA1702.4392775E-618.2616267
TCGACCG2105.0509698E-818.06995431
CGACCGT2753.7380232E-916.3077632
CCCGTAT2352.8383784E-614.8613581
GTCGCGT2101.8171262E-514.78321565
TCGCGTA2152.2692499E-514.4394266
GTCGTTT6500.013.9696761
GTCCGTT5409.094947E-1212.2881411
CGATCGG2255.3744664E-412.26428741
GTCGGTT3205.1486604E-612.005191
GTCGTTA3852.6332418E-711.7925831
CGCCTAT2408.3274994E-411.6413961
TTCGACC3308.319559E-611.49906230
GCGATCG2701.9170897E-411.49776840
GCCTAAC3651.7724033E-611.4819251
GCGTAAC3401.1602393E-511.15987868
GTCCGCT4401.2320015E-711.1122421
CGCGTTT2200.00576549911.1122421
CGATATA2802.6699188E-411.0946164
CGACTGT6251.4551915E-1111.03785939