FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_210_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_210_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4019341
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAC6850.012.12726460
GGCGGTA6057.2759576E-1211.442677559
CGGTACA6007.8216544E-1110.96181861
GAATCGT7051.4551915E-1110.2573364
GTAGTCG4502.1847136E-610.00713957
CGGGAAT6453.0631782E-99.674271
GTCTAAG7657.021299E-109.02403944
TAGTCGC5059.561707E-68.91078458
TGAATCG5503.1643594E-68.7642683
AATCGTC5908.6491673E-78.7542035
ATTCTCG3150.0089223568.7449396
CTCGATT4351.7256553E-48.72646549
AGTCGCC5151.1621244E-48.06542259
AATCGGA7707.0689566E-88.0493185
CTCACTA9851.5279511E-108.0402387
TCTCGAT4754.4597266E-47.991605348
ACACACG9102.0972948E-97.975598352
CTAAGTA10456.184564E-117.92763546
ACACGGG10506.548362E-117.902798754
GTCTCAA10659.822543E-117.760559621