FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_210_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_210_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4019341
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG2401.23473E-817.24747731
GTCGCAT3208.0035534E-1116.3806651
CGACCGT2552.6084308E-816.23292232
GTTCGAC3058.6401997E-1015.8337529
GTCGGTT2503.1779382E-715.3759841
GTCCGAT5700.014.7138611
CGATCGG2657.125891E-714.31723541
GTCCGTT4951.8189894E-1213.4133941
GTCGGTA4701.2732926E-1113.20968262
AGTTCGA4002.8157956E-912.93464328
CCCGAGT4355.8935257E-1012.8534881
TCGACGC3508.956704E-812.81113643
CCGGTAT3559.087489E-812.7969151
TTCGACC3902.7399437E-812.38280530
CTCGGTT5950.012.3336771
GTCCGGA4358.074494E-912.0501451
GCGATCG3257.0304886E-611.67434440
GTCGAGA3003.493484E-511.6484731
GGCGTTT6950.011.061861
TCGGTAA6251.4551915E-1111.03742463