FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_209_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_209_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6921213
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGAT9100.013.27330948
GATTCCG9600.012.59841352
CTCGATT10100.012.30080949
ATTCCGT10800.011.51854953
TTCTCGA10650.011.01707147
TCACCCG13100.010.77663
TCGATTC11600.010.41396450
CGATTCC12400.010.30714851
CCGGACA13850.09.19659412
TCGCGAA3150.0089403918.74294315
CCCGGAC14850.08.57785211
GCGTAGT7051.4753823E-78.30378418
GTTGCGT7951.2167584E-77.794416415
CTCGAAT6351.9908928E-57.6409531
TCGTCGG6352.1062733E-57.608636448
CGGCCCG19550.07.5734558
CGTGGGT15100.07.57039757
GCCCGGA20300.07.462889710
TGCGTAG7951.0029271E-67.363733317
TTTCTCG15950.07.139969346