FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_209_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_209_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6921213
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT5500.017.1453551
TCGACCG2904.2200554E-1016.65332831
GTCGCGA2251.8054834E-615.5225431
CGCGTAA3654.3655746E-1115.11953667
TCGCGTA4052.3283064E-1013.62624866
GTCCGGT11600.012.9466041
CGACCGT3751.5843398E-812.87857232
GTCGTAT6700.012.5107071
GTCGGAT8450.012.3996651
CCGGCTA5850.012.38154337
TCGGATA4501.5865226E-811.5132012
GTCCGTT8050.011.2803571
TCGAATA5906.002665E-1111.1229242
GTCGTTT11100.011.0126151
CCCGAAT8300.010.9405881
GTCGAGA4501.6801641E-710.865781
GTCGCGT4451.7115053E-710.8512465
GCCAGAT30300.010.8350441
TCGGATT10250.010.7830962
GTCGCAC10500.010.644031