FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_301_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_301_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10100891
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAAT49700.07.7983031
GGCGGTA13500.07.41045759
ATTCTCG10051.4893885E-87.2082846
CTCGATT9201.776225E-77.12341949
CGGAAAT12453.0013325E-107.011391
TCATTCG12201.8335413E-96.7873963
CGGTACA13757.4578566E-116.77438461
GTTAAAT45300.06.62878231
GTCGGAT9552.0277403E-66.58116251
GTCGTAT6954.5285551E-46.5311861
GCGGTAC15804.9112714E-116.332127660
GAATCGA10451.6033064E-66.2731994
AATCGTA7702.6474288E-46.2730755
CGGTAAT6150.00604839066.24526741
GGAAGAT104400.06.1539051
GGAAAAT93750.06.1453441
GTTCGAT12601.328408E-76.09657051
CGGGAAT15601.1586963E-96.0432791
AATCGAG11451.1888515E-66.0265355
CGAGAAT11651.3083118E-65.9942871