FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_301_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_301_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10100891
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT7550.013.4015821
GCCGTAT10550.013.2285591
GTCCGTT14050.011.1748361
GTCCGAA10200.010.6039211
GTTCGAT12900.010.5482381
GTCGGAT10750.010.3859561
GTCCGAT11100.010.3727981
GTCCGCT15500.010.3545471
GGCGTTT16550.09.9084291
GTCGCAT7655.638867E-119.5777351
GTTCCGT12500.09.4901681
TCGCATA6252.0228981E-89.3885742
GGCCTAT12400.09.2853281
CCCGAAT9450.09.2302441
GTCGAAA7201.8571882E-99.2071691
GTCGGTA10250.09.1906211
GGCGTGT10650.09.1730421
GTCGGGA13400.09.1131441
GTCCGCA14300.09.0275651
GTCGTAT9701.8189894E-128.9923511