FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_296_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_296_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8557307
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT7800.012.82358249
TCTCGAT9950.010.74795248
TCGATTC10500.010.18490650
ATTCCGT10550.09.15540453
GATTCCG10550.09.15540452
TTCTCGA10450.08.9132247
ACGCGTA3100.00770441078.90324869
GTCTAAG14000.07.88467444
CTCGAAT8554.0015948E-87.75361161
CGGTACA13751.8189894E-127.27625461
TGGGACG27450.07.2451781
CGCCGTA5300.00144166987.15955846
GGCGGTA14001.6370905E-116.899533359
GAATCGT10251.6124795E-76.7329444
GCGATCT7301.3222986E-46.6176543
TTCCGAT10502.4726432E-76.57621152
TCACCCG17900.06.55427653
CGATTCC14806.548362E-116.526318651
ATCGGAT8803.4209108E-56.27296266
TAGTCGC6650.0022477666.225288458