FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_296_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_296_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8557307
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT1657.087874E-821.1328181
TCGACCG5300.013.66632931
CTCGATT9450.012.9144981
CCGGTAT7400.012.2513231
CTCGTAT13200.011.623051
GTCGGTT7400.011.3089131
CGACCGT6008.367351E-1110.92228132
GTTCGAC7300.010.86779229
CCCGTAT8450.010.7289691
GTCGGAT11150.010.6327451
CCCGAAT10850.010.6053631
CGCTAGT2700.002255946210.3315991
CTCCGTT21400.010.2652171
TCGGTTA5151.161261E-710.0546212
GTCCGTT10850.09.9626131
GTCGTTT15550.09.8665121
CTCGTTT23750.09.836771
CTCCGAT15700.09.7722451
TCGGATA6101.3582394E-89.6205692
GTCGGGA12700.09.6096081