FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_295_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_295_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2629838
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGCGC1450.005229180714.27094829
TCTCGAT3104.1428702E-612.24074848
CTCGATT2902.824238E-511.89290949
GATTCCG3652.5416695E-510.39585652
TCGATTC4159.53766E-69.974936550
GGCGGTA3250.00105830299.55346459
AATCGAA4006.921576E-59.4896485
CGATTCC4452.1814958E-59.30229251
CAACTAG4109.1194874E-59.2530888
CCGGACA4109.123272E-59.25273612
CAATCTA4502.4790063E-59.2020824
TTCTCGA3753.8063253E-49.19910747
TTTAACG3200.0099347968.6305652
GTTTATT14300.08.5462831
GTTTAAT13650.08.1858271
GCGGTAC3800.00437423268.17134860
TTTCTCG4855.5964565E-47.82398346
AGATTAG4956.938306E-47.6684024
CCCGGAC5402.0999848E-47.663882711
ATTCCGT4650.00320136437.418352653