FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_295_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_295_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2629838
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT2155.797665E-817.8527681
CGCCTAT1200.001369223317.4470211
GTCGCTA2101.6291217E-514.9545911
CGTCGCG1901.2772821E-414.5334433
CCGGTTA1750.001130676613.807822
CCGCTAT1500.00651059913.806773
CGGATCG1500.006542660813.79653238
CCCGGTT3556.2500476E-913.7610321
GTCGAAT2801.1628708E-613.7083751
GTCCGCA3354.336289E-813.5409721
GTCGCTT3559.25902E-812.7781011
GTCGTAA2551.0104716E-412.3155451
TCGACCG2255.376836E-412.263350531
GTTCCGT3154.358708E-612.1852221
GTCCGGT3802.2461427E-711.9374361
GTTCGTT4104.6809873E-811.9150391
GGGGTAT5601.8189894E-1111.839051
CGACCGT2050.003736377711.77730432
GGCGTTT3651.7901402E-611.4720131
GTCGCAC3358.656067E-611.4577461