FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_259_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_259_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6766911
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA11050.012.48925961
CTCGATT8050.012.42592649
GGCGGTA10950.011.34197959
TCTCGAT9850.011.20706148
GATTCCG10400.010.61398852
GCGGTAC12950.010.3903660
TCGATTC11150.010.2098750
TTCTCGA10600.010.08866847
GTCTAAG13050.010.04428344
TCGCGAA2900.00446221479.51451233
CGCGGTA4754.6042805E-69.43946717
TCACCCG13000.09.024093
CGATTCC13400.09.01000851
GTCGTAT5058.604027E-68.9856771
GCGTATA5301.8389952E-58.46071239
ATTCCGT12050.08.30181253
CCCGGAC14400.08.14331711
TAGCATA18550.07.9946569
ACACGGG19150.07.745430554
ACGGGCG15750.07.66645156