FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_259_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_259_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6766911
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGAT8250.019.450981
TCGACCG4900.018.30123531
CGACCGT5700.016.94296832
GTCCGTT7950.016.2357441
GTCCGCT9500.014.6883811
TTCGACC6350.014.12221330
GTTCGAC6250.013.79682529
TCGGTTA3608.858478E-913.4244932
GTCGGTT5409.094947E-1212.2743191
TCGACGC7100.012.1458143
TCTCGAC7250.011.89451841
GTTCGCA4409.818905E-911.8925811
GTCGATT6351.8189894E-1211.5367411
GTCGCAT5803.6379788E-1111.42781451
GTCCGTA8250.011.416881
GTCGTGA5904.9112714E-1111.2341221
CCCGTAT5301.1314114E-911.1894981
AAGTTCG7450.011.11153827
ACGACCG2802.688446E-411.08657236
AGTTCGA7850.010.98473428