FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323580_258_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323580_258_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23410410
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG11050.014.04615331
GTCGGTT19800.013.2123411
CGACCGT13100.012.11138232
GTCCGCT29400.012.1014271
GTCGAGA19500.011.2691131
GTCGTTT37500.011.1617871
GTTCGAC14600.010.86764129
GTCGCAT17800.010.3857921
GTCCGAT33150.010.3116051
GGCGTTT37850.010.0448711
CCCGTAT18650.09.7254171
GTCCGAA22350.09.6760451
GTCCGTT28200.09.647821
GTCGTAT25500.09.4382761
GTCGGGT35850.09.4377031
CCGGCTA21250.09.41428637
GTTCGAT45050.09.3685921
GTCGGCT37300.09.3513631
GTCCCGT44900.09.3222061
GTCCGGT40400.09.1518361